External Funding - At a Glance
ERC (European Research Counsil)
- DISSECT - Disseminating tumour cells as novel biomarkers: Dissecting the metastatic cascade in cancer patients
- CTCapture_2.0 - Advanced platform for profiling of therapeutic targets and functional analysis of circulating tumour cells in cancer patients
EU (European Union)
- CANCER-ID - Cancer treatment and monitoring through identification of circulating tumour cells and tumour related nucleic acids in blood
- ELBA Marie Curie Innovative Training Network (ITN)
EU / BMBF (The Federal Ministry of Education and Research)
- PROLIPSY TRANSCAN-2 - Early Detection of Prostate Cancer by Liquid Biopsies
- TRANSCAN CTC-SCAN - Circulating Tumor Cells as Biomarker for Minimal Residual Disease in Prostate Cancer
BMBF (The Federal Ministry of Education and Research)
- Ci3 : Development of a Microfluidic-Based System for Enrichment and Single Cell Analysis of Circulating Tumor Cells (CTCs)
DFG (German Research Foundation)
Functional Characterization of the Putative Metastases Suppressor Protein RAI2
Klaus Pantel, PI
Harriet Wikman, PI
Andreas Trumpp, Deutsches Krebsforschungszentrum (DKFZ)
Please visit the project site of the DFG [German/English].
The onset of the metastatic cascade -the main cause of cancer-related death is the dissemination of single tumor cells into distant organs. The presence of disseminated tumor cells (DTCs) in the bone marrow (BM) of cancer patients is an independent prognostic factor for the occurrence of metastatic relapse in different epithelial tumors.
In the preceding funding period we were able to identify genes in breast, colon and lung cancer patients, which were associated with DTC status. One of the identified candidate genes is the putative metastasis suppressor gene retinoid acid induced gene 2 (RAI2) with largely unknown molecular function. RAI2 was found to be significantly down regulated in primary tumors of BM-positive patients. Additionally low RAI2 expression was correlated with a significant decreased survival of breast, lung and ovarian cancer patients. Subsequent cell culture-based functional analyses and bioinformatical analyses revealed that up-regulation of the RAI2 transcript is associated with the induction and/or regulation of senescence. Thus, our current results imply that deficient senescence in the primary tumor sustains the dissemination of tumor cells. We assume that the deregulation of RAI2 protein expression might also be important for metastatic outgrowth or escape from dormancy.
The primary goal of the applied project is a comprehensive functional characterization of the RAI2 gene product in the context of tumor cell dissemination. Furthermore we want to investigate whether RAI2 could present a target for the development of future therapeutic strategies for the treatment of cancer patients, e.g. dormancy control.
Deutsche Krebshilfe (German Cancer Aid)
- „Preventive Strategies against Brain Metastases“, German network
- „DETECT-CTC: Detection and molecular characterization of circulating tumor cells and cell-free nucleic acids in advanced breast cancer in the context of tumor heterogeneity”, German network
- Hamburger Krebsgesellschaft (Hamburg Cancer Society)
- Erich und Gertrud Roggenbuck-Stiftung (German Foundation dedicated to advancing cancer research)
- Stiftung für das Leben (German Foundation for Life)
- Hiege-Stiftung gegen Hautkrebs (German foundation against skin cancer)
- Forschungsförderungsfonds der Medizinischen Fakultät Hamburg (FFM)
(Research Fund of the Medical Faculty of the University of Hamburg)