SynTagMA, a new tool to label high Ca2+ spines. Green are inactive spines, magenta active, cyan blobs are the presynaptic terminals we activated with Channelrhodopsin. Silver spheres are regions of interest, automatically detected. This piece of tissue is alive, not fixed! Perez-Alvarez A et. (2019) Freeze-frame imaging of synaptic activity using SynTagMA. bioRxiv, DOI 10.1101/538041
New neuron-specific optogenetic tools
We have deposited a number of neuron-specific optogenetic tools at Addgene.org including
We have also deposited a floxed construct for selective inhibition of cre-expressing cells ( Plasmid #70762 ).
Other published constructs are available on request.
Information on current research
D. Evanko (2014) A better way to turn off neurons . Highlight in Nature Methods 11(6):608.
E. Pastrana (2011) Perfecting ChR. Research Highlight. Nature Methods 8(6):477.
Function of neglected structure In neurons revealed after 50 years . ScienceDaily, 8 September 2009.
J. C. Lopez (2002) From single lane to superhighway . Research Highlight in Nature Reviews Neuroscience 3:588.
In the new DFG Priority Programme 'Optogenetics', Simon Wiegert and Peter Soba recieved funding for the developement of novel optogenetic tools in Drosophia. Congratulations!