If you work with mouse models and require histological analysis of tissues relevant to your research, we are happy to assist. Depending on your specific research question, we can perform analyses that either map the expression patterns of various proteins and their spatial distribution within the tissue, or reveal histological differences between your individual mouse model and wild-type tissue. For investigations at the nanoscale, the core facility can also visualize individual cells using electron microscopy. The following services are available:

• Production of paraffin and Kryoblöcken (with decalcification): see protocol for sample preparation
• Preparation of cell blocks
• Cutting of paraffin and Kryopräparaten
• Histological staining (z. B. with hematoxylin and eosin, Masson Goldner, Elastica van Gieson, etc.)
• Immunohistochemical staining (z. B. Ki67, Caspase-3, Iba-1, GFAP, CD-3, B220, etc.)
• Establishment of an antibody for Immunohistochemical Staining
• Production of a preparation for electron microscopy: see protocol for sample preparation
• Creating electron microscopic images
• Quantification of cell populations in tissues

The facility is open to all interested researchers, in accordance with the user regulations. A flat fee applies for the services provided.
For further information, please contact Dr. Krasemann. If you wish to submit samples for analysis, please follow the procedure described below:

• Contact the Core Facility prior to sample collection, in order to ensure an ideal sample preparation
• Use for its mission the prefabricated form
• Samples are accepted by appointment.
• Samples can be used in the form of fresh tissue, fixed tissue or be proposed as a prefabricated block of tissue.