NEW INSIGHTS FROM 3D ELECTRON MICROSCOPY
Does it change the Cell Biology Textbooks?
Meeting with panel discussion was postponed!
Erika-Haus, UKE Campus Building W29, Martinistr. 52, Hamburg ( Map )
Ruben Fernandez-Busnadiego, Uni Göttingen
Kay Grünewald, CSSB Hamburg
Frederic Leroux, Leica Microsystems Diegem
Robert Kirmse, Leica Microsystems
Emine Korkmaz, Thermo Fisher Scientific
Rudolph Reimer, HPI Hamburg
Alex Rigort, MPI Martinsried
Michaela Schweizer, ZMNH Hamburg
After the Meeting you may visit the Facilities at
ZMNH Core Facility Morphology and Electron Microscopy
Our facility covers a large spectrum of light and electron microscopy techniques with a major focus on sample preparation and characterisation of genetically engineered animals, including immunolocalisation of proteins, ultrastructural analysis, and data processing.
We advice interested scientists on morphological questions and teach and train researchers in the application of microscopy techniques. Finally, our facility introduces and establishes new techniques and guarantees efficient use of the respective equipment.
• Performance of light- and electron microscopical investigations
• Advice and practical instruction in the application of histochemical techniques
• Instruction of researchers in operation of microscopes and accessories
• Introduction of useful new (immuno-) histochemical techniques and/or equipment
• Morphological studies of many kinds of tissues with light-, confocal laser scanning-, or transmission electron microscopy
• Patho-histological analysis of the whole body of transgenic mice
• Histo- (cyto) chemical staining procedures
• Immunohisto- (cyto) chemistry
• Pre- and postembedded immunogold labelling techniques
• Correlated light-and electron microscopy (CLEM)
We prepare cell and tissue samples for scientific histological and (immuno-) histochemical light and fluorescence microscopy. All preparation steps, (including fixation, sectioning with vibratome, cryotome or microtome, staining, mounting etc.) are performed by the group.
The Morphology Unit has at its disposal both conventional and fluorescence microscopes (Zeiss
Axiophot), as well as two confocal scanning laser microscopes in inverted (Leica SP2) and in
upright configuration (Olympus Fluoview 1000). We process cells and tissues for conventional
transmission electron microscopy (Zeiss 902) and offer immunolocalisation of gene products
applying pre- and postembedding protocols. We take care to preserve both antigenicity and structural integrity. All results are documented in high resolution digital images.
Dr. Michaela Schweizer, Group Leader