Transgenic animals
The transgenic mouse facility of the ZMNH supports scientists of the UKE in all aspects of transgenic mouse production.
We offer as service:
- Help with the design of experiments
- The generation of transgenic mice by pronucleus injection of DNA constructs into one cell stage mouse embryos
- The injection of recombinant mouse ES cells into mouse blastocysts
- ES cells and mouse embryonic fibroblasts for ES cell culture
- Supervision of gene targeting experiments carried out in the cell culture laboratory of the facility
- Mouse lines of general interest (Cre-, Flip-Deleter and reporter mice)
- Help with the preparation of DNA for pronucleus injection and electroporation into ES cells
- Help with the analysis of recombinant ES cell clones as well as of the generated mouse lines
- Embryo transfer of mouse lines carrying more than one transgene
We work in close collaboration with the VTH.
For further information please contact:
PD Dr. Irm Hermans-Borgmeyer
e-mail: ihermans@uke.uni-hamburg.de
Phone: 56663
